EcoRI/PstI Restriction Enzyme Mix-(ELS-ELE1518)

Bio-Rad EcoRI/PstI Restriction Enzyme Mix 3000 units - DNA evidence can be used to identify a perpetrator or exonerate the innocent. With this kit, students use real DNA as evidence and play the role of crime scene investigator. Six DNA samples are included: one sample has been collected from a “crime scene” and five others from various “suspects.” Each sample is digested using a mixture of two DNA restriction enzymes to generate a distinct set of DNA fragments for each sample. The resulting DNA fragments are separated by agarose gel electrophoresis and visualized to determine “whodunit”! - Real-world forensics — open the door to rich discussions about the scientific, ethical, and legal implications of forensics, DNA profiling, and genetic engineering Uses real DNA — the DNA samples provided are engineered to mimic the natural variations in DNA that exist between one human being and another Fast results — after restriction digestion, separate the fragments in as little as 10 minutes Optional sizing activity — guide students through the construction of a standard curve using their own gel data, then challenge them to apply their math skills and use their standard curve to estimate the molecular weights of the fragments generated by different restriction enzymes Finish all activities in two 45-minute class periods Consumable reagents kit includes DNA samples, DNA standard, restriction enzyme mix, sample buffer, and sterile water; for educational use only